Luminita-Cornelia Andrei-Selmer, PhD, PM

Interes stiintific

Identicarea interactiilor in care o proteina e implicata si activity-based protein profiling in identificarea proceselor biochimice au semnificativa valoare in era post-genomica. Ma intereseaza cercetarea aplicata in scopul identificari de biomarkeri si selectia de efector specifici – de exemplu peptide, anticorpi sau celule modificate pentru scopuri terapeutice. Ma intereseaza cartarea proteinelor cu rol specific, interactia cu alte molecule biologice si evaluarea interactiilor cu modulatori sau substrate, pato-fiziologia si aspectele clinice. In cercetarea fundamentala si translationala (cercertarea biomarkerilor si terapia imunitara) aplic technici de visualisare in vivo si analiza interactiilor moleculare in vitro – basata pe afinitatea naturala a proteinelor.

a. Functia si topografia disintegrin proteazelor si substratele specifice in ontogeneza. [i]

b. Dezvoltarea mijloacelor de evaluarea a biomarkerilor pentru Alzheimer’s diseases, si implicarea in terapia imunitara[ii], [iii], [iv].

c. Rolul junction adhesion molecules in inflamatie si in cellule canceroase de plaman in metastaza[v].

d. Interactia specifica intre neutrophile (via antigenului specific CD177) si endothelium (via CD31 membranar)[vi]. Interactia heterophilica CD177-CD31 are loc in inflamatie si este mai puternica decat interactia homophilica CD31-CD31. Expresia CD31 controleaza expresia si activitatea HO-1. [vii]

e. Analiza serului pacientilor cu neonatal alloimmune mediated thrombocytopenia [viii], [ix]

Projectele citate au implicat cooperarea cu institute de cercetare academice si din industria farmaceutica si biotechnologie.

[i] Date nepublicate.

[ii] R. Dodel, K. Balakrishnan, K. Keyvani, O. Deuster, F. Neff, L. C. Andrei-Selmer, S. Roskam, C Stuer, Yl-Abed, M. Balzer-Geldsetzer, W. Oertel, Y. Du and M. Bacher, Naturally occurring autoantibodies against ß-amyloid: investigating their role in transgenic animal and in-vitro models of Alzheimer’s disease, J. Neuroscience 31(15): 5847-54, (2011)

[iii] D. Bartnik*, S. A. Funke*, L. C. Andrei-Selmer*, M. Bacher, R. Dodel and D. Willbold, Differently Selected d-Enantiomeric Peptides Act on Different Abeta Species., Rejuvenation Res. 13(2-3): 202-5, (2010)

[iv] K.Balakrishnan, L.C. Andrei-Selmer, T. Selmer, M. Bacher and R. Dodel

Comparison of intravenous immunoglobulins for naturally occurring antibodies against β-amyloid,  J Alzheimers Dis. 20(1):135-43, (2010)

[v] S. Santoso, V. Orlova, K. Song, U. J. H. Sachs, C. L. Andrei-Selmer, T. Chavakis, The homophilic interaction of JAM-C mediates tumor cell-endothelial cell interactions. J Biol Chem. 280, 36326-33, (2005)

[vi] U.J.H. Sachs, L. C. Andrei-Selmer, A. Maniar, C. Paddock, P.J. Newman, T. Chavakis, and S. Santoso The neutrophil specific antigen CD177 is a novel counter-receptor for endothelial PECAM-1 (CD31). J. Biol. Chem. 282 (32): 23603-12, (2007)

[vii] H. Saragih, E. Zilian, Y. Jaimes, A. Paine, C. Figueiredo, B Eiz-Vesper, R. Blasczyk, J. Larmann, G. Theilmeier, L. C. Andrei-Selmer, J Becker, S Santoso and S Immenschuh

PECAM-1 dependent heme oxygenase-1 regulation via a Nrf2-Mediated Pathway in endothelial cells, Thrombosis and Haemostasis, 111(6): 1077-88, (2014)

[viii] Socher I*. L. C. Andrei-Selmer*, G. Bein and S. Santoso, Low-avidity HPA-1a alloantibodies in severe neonatal alloimmune thrombocytopenia are detectable with surface-plasmon resonance technology, Transfusion 49(5): 943-52, (2009)

[ix] S. Santoso, H. Kroll, L. C. Andrei-Selmer, I. Socher, A. Rankin, E. Kretzschmar, N. A. Watkins, W.H. Ouwehand

A naturally occurring Leu₃₃Val mutation in beta3 integrin impairs the HPA-1a epitope: The third allele of HPA-1,

Transfusion 46 (5), 790-9, (2006)

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* contributie egala

Afiliere

Centrul OncoGen, Timisoara