Decellularization and recellularization of the heart
An important role of cardiac stem cell research is the engraftment of beating cardiac cells into the ischemic region of the heart after a myocardial infarction or more recently for the recelullarization in vitro of the decelullarizated heart for the reconstruction of a bioartificial heart. Decellularization is a procedure which involves chemical, physical or enzymatic treatment of an organ or tissue to eliminate all resident cells in order to obtain an extracellular matrix. The aim of this procedure is to obtain an intact extracellular matrix of an organ or tissue which can be used in regenerative medicine, transplantation and/or bioartificial organs. In our experiments we have used and compared different methods of decellularization and we have differentiated stem cells into cardiomyocytes and other cell types in order to establish the best protocol for decellularization and recellularization of the heart.
1.Ioana Mihaela Citu, Florin Borcan, Csilla Zambori, Bogdan Tita, Virgil Paunescu, Simona Ardelean, Influence of Crosslinking Agent – Chain Extender Ratio on the Properties of Hyperbranched Polyurethane Structures used as Dendritic Drug Carrier, REV. CHIM., 66, Nr. 1, pp. 119 – 123, 2015, Bucharest, ISSN 0034-7752.
UMF “Victor Babes” Timisoara
Oncogene Institute -SCJUT Timisoara